General cell culture:
Supports the growth and maintenance of a variety of human and animal cell lines, including primary cells, fibroblasts, and tumor cell cultures.
Tissue and organ culture:
Due to its inclusion of nucleosides and coenzymes (such as NAD, NADP, and Coenzyme A), CMRL-1066 is suitable for short-term maintenance of mammalian tissue fragments or organ explants in vitro.
Low-serum or serum-free systems:
The presence of ascorbic acid, glutathione, cholesterol, and Tween 80 helps maintain membrane stability and cellular vitality under low-serum conditions.
Hence, it is frequently used in the development of serum-free or chemically defined culture systems.
Host–pathogen co-culture studies:
As a nutritionally rich basal medium, CMRL-1066 is often employed for the co-culture of cell-dependent microorganisms such as Mycoplasma, Rickettsia, or certain viruses.
Cell recovery and pre-freezing maintenance:
With multiple antioxidant and metabolic support components (e.g., glucose, niacinamide, FAD, glutathione), it is also used for short-term recovery culture prior to cryopreservation or thawing.
· Enhanced Lipid Supplementation:
Incorporation of soluble lipid components improves cellular and mycoplasma metabolism, optimizes membrane composition, and enhances stress resistance—resulting in superior culture performance.
· Stable Physiological Buffering:
Formulated with 20 mM HEPES, maintaining pH precisely at 7.4 to ensure consistent environmental stability throughout incubation and handling.
· Serum Protein Enrichment:
Addition of defined serum proteins enhances culture stability and reinforces overall environmental consistency for reproducible results.
· Simplified, Indicator-Free System:
Sodium bicarbonate and phenol red have been removed to reduce variability and eliminate potential optical or chemical interferences during sensitive assays.
· Optimized Amino Acid Profile:
L-glutamine and L-cysteine are excluded to minimize degradation-related byproducts and maintain long-term formulation stability.
· Light-Protective Packaging:
Supplied in amber bottles to preserve the integrity of light-sensitive components and ensure sustained biochemical activity during storage.
Inorganic Salts |
Calcium chloride dihydrate、Magnesium sulphate anhydrous、Potassium chloride、Sodium acetate anhydrous、Sodium chloride、Sodium dihydrogen phosphate anhydrous |
Amino Acids |
Glycine、L-Alanine、L-Arginine、L-Aspartic acid、L-Histidine hydrochloride monohydrate、L-Glutamic acid、L-Cystine、L-Isoleucine、L-Lysine hydrochloride、L-Leucine、L-Phenylalanine、L-Methionine、L-Proline、L-Threonine、L-Serine、L-Tryptophan、L-Tyrosine disodium salt dihydrate、L-Valine、Trans-4-Hydroxy-L-Proline |
Vitamins & Cofactors |
Choline chloride、D-Biotin、D-Pantothenate (hemicalcium)、Folic acid、Folic acid、L-Ascorbic acid sodium salt、Nicotinamide、Nicotinic acid、Pyridoxal hydrochloride、Pyridoxine hydrochloride、Riboflavin、Thiamine hydrochloride、myo-Inositol、p-Amino benzoic acid (PABA) |
Nucleosides & Derivatives |
2′-Deoxyadenosine、2′-Deoxycytidine hydrochloride、2′-Deoxyguanosine、5-Methyldeoxycytidine、Cholesterol、Cocarboxylase、Coenzyme A sodium salt、D-Glucose、D-Glucuronic acid sodium salt、FAD disodium salt、Glutathione (reduced)、Thymidine、Uridine-5-Triphosphate sodium salt、β-NAD、β-NADP sodium salt Lecithin |
suitable for microbiology, plate diam. 60 mm, For general mycoplasma detection, pack of 10 plates，box of 10 packs
suitable for microbiology, plate diam. 60 mm, For general mycoplasma detection, pack of 10 plates，box of 10 packs
suitable for microbiology, plate diam. 60 mm, For general mycoplasma detection, pack of 10 plates，box of 10 packs
suitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detectionsuitable for bioburden testing, For mycoplasma detection
suitable for bioburden testing, For mycoplasma detection
suitable for bioburden testing, For mycoplasma detection
